Creating a DVD with 5.1 mix from flattened quicktime file
 

If one has a flattened quicktime file and wants to create a DVD with a 5.1 mix from it, using compressor or adobe media encoder.

What does that flattened .mov file need to have audio wise? If it has 6 discrete channels, can I then take that, and create a 5.1 mix with one of the above programs? Or does the MOV file need to be 5.1 from the start?