I second this: "Yippee-ki-yay, motherf***er." Die HArd 1988
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Here are some more:
"you think that's air you're breathing now?" The Matrix (my fav kick azz movie)

"Agent Smith : You were given _specific_ orders...

Lieutenant : Hey, I'm just doing my job. You give me that

juris-my-dick-tion crap, you can cram it up your ass. " The Matrix

''Neo: I Know Kung Fu" The Matrix

"Mouse : That's exactly my point. Exactly. Because you have to wonder
now, how did the machines really know what Tasty Wheat tasted
like, huh? Maybe they got it wrong. Maybe what _I_ think
Tasty Wheat tasted like actually tasted like, uh..oatmeal, or
tuna fish. That makes you wonder about a lot of things. You
take chicken for example, maybe they couldn't figure out what
to make chicken taste like, which is why chicken tastes like
everything. Maybe they couldn't... " The Matrix

"Mouse : The woman in the red dress! I, I designed her. She, um..well
she doesn't talk very much, but...but if you'd like to meet
her, I can arrange a much more personalized milieu.
Switch : Digital pimp, hard at work. " The Matrix

"Hey! You taste it you buy it!" -Eating Raoul

"It's not the years, it's the mileage." -Raiders of the Lost Ark

"How long could we maintain? I wondered. How long until one of us starts raving and jabbering at this boy? What will he think then? This same lonely desert was the last known home of the Manson family; will he make that grim connection when my attorney starts screaming about bats and huge manta rays coming down on the car? If so, well, we'll just have to cut his head off and bury him somewhere, 'cause it goes without saying that we can't turn him loose. He'd report us at once to some kind of outback Nazi law enforcement agency and they'll run us down like dogs. Jesus, did I say that? Or just think it? Was I talking? Did they hear me?" -Fear and Loathing in Las Vegas

"Your miserable life is not worth the reversal of a Custer decision." -Little Big Man

"What we have here is failure to communicate." -Cool Hand Luke

My vote for a well known movie line that has never actually worked...

"Good-bye mister Bond."

Favs include, in no particular order...

"I'm a great lover cuz I practice so much by myself." Woody Allen, Love and Death

"I think you smoked yourself retarded" from Halfbaked

''You can milk anything with nipples.'' Meet the Parents

''I'm looking for Ray Finkle." Someone puts a gun to his head. "And a clean pair of shorts." Ace Ventrua